![]() ![]() Understanding basic imaging concepts such as sensitivity, resolution, and sources of. Accurate imaging of your western blot is crucial for capturing blot data for downstream analysis. Dynamic Range of 4.82 orders of magnitude. Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights.True Color Imaging of translucent and opaque samples.Capture images of visible fluorescence WB in multiplex, ECL WB, biomolecules tagged with fluorescent labels in gels, fluorescent and colorimetric stains in gels, Petri dishes, TLC, etc.Upgradeable to include two Near-Infrared fluorescence channels – The Azure 400 can be field upgraded to the Azure 600 for applications detecting NIR700/NIR800 or similar fluorochromes.Indicates eventual signal saturation for accurate quantitation. Fast and Sensitive Chemiluminescent Detection – Outperforms film performance while generates a quantitative, wide dynamic range image.Add Tween 20 detergent to the wash buffer to a final concentration of 0.05. Increase the number of washes and/or the volume of buffer used. Automatic Saturation Detection highlights saturated. Use Thermo Scientific SuperSignal Western Blot Enhancer to reduce background and enhance detection of low-abundance and weakly immunoreactive antigens. Analyze single channel or multichannel images with options to view individual channels in a multiplex image, view all channels simultaneously, or delete channels. Three RGB fluorescence channels – For applications detecting fluorescent proteins in the visible range, Cy2/C圓/Cy5 or similar fluorochromes. Analysis of in-cell Western blots, Western blots, and more are made easy with AzureSpot Pro.Connectivity through WI-FI, Ethernet, and USB in a Microsoft Windows10 OS. Use the preset protocols of the comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperture, exposure time, focus, distance to the camera, and resolution. Intuitive capture software controlled by a wide touchscreen. Obtain your images immediately and be ready to quantify bands in the analysis software. The imaging chamber replaces the darkroom and film developers to generate a digital image including true color molecular weight marker. ![]() Chemiluminescence detection that surpasses X-ray film detection in sensitivity and dynamic range. Image and quantify up to three different targets simultaneously. The three-channel Visible Fluorescence detection enables sensitive multiplex detection of WBs, fluorescent biomolecules and Cy2/C圓/Cy5 or similar fluorochromes. The most flexible solution to detect three fluorescent targets in the visible range in multiplex, ECL WBs, and do all your gel documentation. This pre-processed Western blot was developed as a tool to verify the performance of fluorescence imaging systems, including laser scanners and CCD-based. Find out about the advantages and disadvantages of different imaging options and identify the most suitable method for your application. ![]() The suitability of an imaging technique depends on the detection method and level of analysis needed for an experiment. Visible Fluorescence Imaging System for Western Blots, Fluorescent Proteins and Stains. Choosing an imaging solution for Western blots. ![]()
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